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FBISE Class 12 Biology Experimentation Skills Book (2025 Edition) – Free PDF Download

September 12, 2025
written by Sidra Batool

The Class 12 Biology Experimentation Skills textbook (2025 Edition) is published by the National Book Foundation (NBF) under the Federal Board (FBISE). It is a supplementary resource designed to improve practical biology learning through real-life experiments, data analysis, and inquiry-based tasks.

This edition includes refined illustrations, hands-on experimental activities, and SLO-based content to help students prepare for practical assessments and model papers. The book strengthens problem-solving, critical thinking, and scientific inquiry skills in learners.

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Table of Contents

List of Experiments in Class 12 Biology Experimentation Skills Book

  • Experiment 1: Investigating bacterial sensitivity to antibiotics (Disc Diffusion / Kirby-Bauer Test)
  • Experiment 2: Applying Chi-Square (X²) Test to analyze genetic ratios in a dihybrid cross
  • Experiment 3: Pearson’s Correlation Test for species occurrence in grasslands
  • Experiment 4: Factors affecting enzyme activity (temperature, pH, enzyme concentration)
  • Experiment 5: Preparation of solutions and serial dilutions from stock solution
  • Experiment 6: Analysis of antibiotic use and misuse through provided data
  • Experiment 7: Measuring leaf surface area variability using graph paper method
  • Experiment 8: Identification of ABO and Rh blood groups with antisera
  • Experiment 9: Determining child’s blood group from parental types (phenotypes/genotypes)
  • Experiment 10: Human height/weight variability in students of the same age group
  • Experiment 11: Effect of fertilizer or soil type on plant growth (height and leaves)
  • Experiment 12: Effect of light intensity on plant growth (height and leaves)
  • Experiment 13: Effect of different water levels in soil on plant growth
  • Experiment 14: Effect of light intensity on rate of photosynthesis

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You can download the Class 12 Biology Experimentation Skills Book (2025) PDF for free or read it online. This book is not for sale and is officially published by the National Book Foundation (NBF) for the Federal Board (FBISE). All rights are reserved by the National Book Foundation.

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